Atma Jaya

Suppressive Effect of Ethanolic Kaempferia Pandurata Roxb. Extract on Matrix Metalloproteinase - 2 Expression in Porphyromonas Gingivalis - treated Human Gingival Fibroblasts in Vitro
Suppressive effect of ethanolic Kaempferia pandurata Roxb.


extract on matrix metalloproteinase-2 expression in


Porphyromonas gingivalis-treated human gingival fibroblasts


in vitro



Yanti1) and Jae-Kwan Hwang2)


1)Faculty of Biotechnology, Atma Jaya Catholic University, Jakarta, Indonesia


2)Department of Biotechnology, Yonsei University, Seoul, Korea


(Received 25 May and accepted 5 October 2010)

In periodontal disease, gingival fibroblasts activated by the Gram-negative anaerobic bacteriumPorphyromonas gingivalis induce overexpression of matrix metalloproteinase-2 (MMP-2), which is involved in inflammatory progression. This process is followed by tissue destruction and bone loss. In the present study, we investigated the in vitro effect of the ethanolic Kaempferia pandurata Roxb. extract on expression of MMP-2 in P. gingivalis-treated human gingival  fibroblast-1 (HGF-1) cells. In addition, we utilized gelatin zymography, Western blotting, and reverse transcription-PCR analysis to elucidate the molecular mechanisms underlying MMP-2 inhibition via the mitogen-activated protein kinase (MAPK) and cyclic AMP response element-binding protein (CREB) signaling pathways. Treatment with K. pandurata extract (1-10 ìg/ml) dose-dependently suppressed the activity, secretion, and protein expression of MMP-2 in HGF-1 cells exposed to P. gingivalis. At the transcriptional level, inhibition of MMP-2 gene expression by K. pandurata was mediated by phosphorylation of c-Jun N-terminal kinase (JNK) and CREB signaling pathways in P. gingivalis-treated HGF-1 cells. These results suggest that K. pandurata extract suppresses MMP-2 expression at the protein and gene levels via downregulation of the principal JNK and CREB signaling pathways. Due to its efficacy in inhibiting MMP-mediated periodontal destruction, K. pandurata might represent a new, potent periodontal therapy. (J Oral Sci 52, 583-591, 2010)

Keywords: Kaempferia pandurata Roxb.; matrix metalloproteinase-2; Porphyromonas gingivalis; human gingival fibroblasts.

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